Lab on cell-free synthetic biology

Course summary

• Announcements (Forum)
• Course schedule (File)
• Upload DNA sequence for your project here (Forum)
• Upload your protocol here (Forum)

---

• Presentation file (File)
• Presentation file (File)

---

• Colony PCR and Agarose Gel Electorphoresis Protocol (File)
• E. coli BL21(DE3) Transformation Protocol (File)
• plasmid sequence (File)
• primer sequence (File)
• Week_2_theory_lab_on_cell_free_expression (File)

---

• Protein Expression in BL21(DE3) Protocol (File)
• Cell-free Reaction Protocol (File)
• CFsynbio_lab week3 (File)
• Results (File)

---

• OnePot PURE Expression Protocol (File)
• Table of PURE Proteins (File)
• Presentation (File)

---

• Protein Purification Protocol (File)
• Buffer Exchange and Concentration Adjustment Protocol (File)
• Presentation (File)

---

• Presentation (File)

---

---

• Ribosome Production Protocol (File)
• Presentation (File)
• Result of Functional Test of OnePot PURE (File)

---

---

• Ribosme funcitonal assay result (File)

---

• Project - first result (File)

---

• Project - second result (File)

---

• Project - third result (File)

---

---