Biomicroscopy II

Biomicroscopy II

The course builds on Biomicroscopy I and combines theory of different microscopy techniques with hands-on experiments on the state-of-the-art microscopes at EPFL BioImaging and Optics Facility (BIOP). The goal of the course is to teach how to use commonly used optical microscopy techniques including brightfield,  dark-field, phase-contrast, DIC, fluorescence as well as introduce advanced fluorescence microscopy techniques such as confocal, super-resolution and multi-photon.

Lectures: 

Where: BC 01

When: Thursdays starting from 20 February at 13:15 (Prof. Hatice Altug)

Exercises: 

Where: BC 01

When: Thursdays after lectures 

Practical:

Where: BioImaging and Optics Platform (BIOP)

When: Thursdays from middle of the semester: starting at 13:15 until 17:00 (Dr. Arne Seitz)

• http://www.olympusmicro.com/
• http://zeiss-campus.magnet.fsu.edu/tutorials/index.html
• http://microscopyu.com/tutorials/

Lecture Part:

• Review of Wide Field Microscopy (Biomicroscopy I)
• Dark-Field Microscopy
• Phase-Contrast Microscopy
  

Lecture Part:

• Phase Contrast Microscopy
• Polarization Optics
• Polarization Microscopy
• Birefringence Optics

Lecture Part:

Differential Interference Contrast (DIC) Microscopy

Fluorescence Microscopy Techniques:

- FRET (Förster resonance energy transfer) technique

- TIRF (Total internal reflection fluorescence) technique

- FRAP (Fluorescence recovery after photo bleaching) technique

Lecture Part:

• FRAP 
• Confocal Microscopy

Lecture Part:

• Advanced Microscopy I 

Lecture Part:

• Advanced Microscopy II

Practical Part:

Introduction to Wide-Field Microscopy

Practical Part:

Transmission Microscopy Techniques -1

Practical Part:

Fluorescence Microscopy - 2

Practical Part: 

Confocal microscopy

Practical Part:
Confocal/Fluorescence Microscopy - Continued

HOLIDAY