Bioprocesses and downstream processing

This session is for you!

In case you have questions about the course content, the examination material or what will be expected from you please join us, raise your hand and ask!

We will do our best to give you the information you might be missing

Fermentation basics (Part 1)

1. Drivers of biotechnology
2. The cell
3. Preservation of cells
4. Batch culture
5. Medium design

Fermentation basics (Part 2)

• Batch calculations
• Fed-batch applications
• Introduction chemostat

(optional start of Part 3)

Fermentation basics (Part 3)

• Continuous culture
  
• Maintenance energy
  
• Multiple nutrient limited growth conditions

Agenda:

Visit of labs in upstream (M. Zinn) and downstream processing (S. Crelier)

Questions concerning paper presentations

Bioprocess paper studies

• 5 Paper presentations by Master students

Introduction and Basics of Bioreactors / Fermentors

- Background

- What are drug products?

- History of modern biopharmaceutical manufacturing

- Using cells to manufacture chemicals

- The birth of modern biopharmaceuticals

- Chose of a Bioreactor / Fermenter

- Characterization of biological reactor systems

- Different types of reactor systems

- Basic geometry of reactors and some basic calculations 

- Transfer phenomena

- Bioprocess mass transfer

- Oxygen Transfer

- Agitator design and operation

- Mixing

- Power Input

- Scale-up of bioreactors / fermenters 

- Sterilisation of Bioreactors / Fermentors

- SUT (Single Use Technology)

- Economic analysis

Anatomy of a bioprocess and overview of DSP in biotechnology

Which biomolecules do we purify? 

Production of mAbs. Some analytical aspects.

The separation of the biomass from the fermentation medium is a mandatory step in DSP

In this lecture we will discuss centrifugation and filtration

This week we will discuss membrane techniques: micro-, ultra-, nanofiltration and reverse osmosis

Chromatography is probably the golden standard approach when it comes to preparative protein purification, be it at lab or production scales. But wait, which type of chromatography are we talking about? How do you assess the performance of your resin/columns? Follow the guide, please!

This section contains a few documents we will discuss during the last lecture.

The documents that will be part of the examination material will be clearly identified.  

Here you will find articles on affinity chromatography and continuous chromatography that you might find useful if you get to work on such systems. 

The Dossier "Cytiva Handbooks" contains a collection of very useful booklets on protein purification and the different chromatography techniques.

The video shows the basic principles behind Simulated Moving Bed Chromatography, a technology that enables continuous chromatographic separations.

In the bottom part of the section you will find three documents dedicated to viral clearance, an important step in the purification of molecules derived from mammalian cell cultures (unfortunately, we did not have enough time to cover this topic).

In this section you will find a few typical DSP questions and short calculations (with answers) to help you prepare the exam of July 3.

In case some topics need clarifying, we will be available to answer your questions during this Zoom session