Chemical Probes for Imaging in Biology (K. Johnsson)

The course focusses on the visualization and manipulation of biological activities in live cells. Topics discussed in the class will be (i) the design of new fluorescent probes for live-cell imaging, including superresolution microscopy; (ii) methods for the direct and specific localization of chemical probes in living cells; (iii) the design of semisynthetic protein sensors for basic research and diagnostics; (iv) methods for labelling of DNA; (v) approaches for manipulating biology processes with chemical probes. The class will be comprised of five lectures of 2 hours each around these topics. At the end of the course, each participant will write a short report of how her or his PhD project and could benefit from the approaches discussed in this course of this class.