FastQCFastQC Report
Thu 25 Feb 2021
6014_hpp_HS_Rep3_S25_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6014_hpp_HS_Rep3_S25_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37121145
Sequences flagged as poor quality0
Sequence length35-92
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATGGATGTGTAGATCTC1013320.272976493586068Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATGGATGTGTAGATCT411250.11078591460473537Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)