Labo intégré en sciences de la vie I

BIO-203

WATCH Standard Protocol Restriction Enzyme Digest

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Description

For optimal digestion it important to use the correct type of mixing.

Questions:

What are the two types of mixing recommended for the reaction?
What type of mixing is NOT recommended?

To save material, we scaled the reaction volume down to 20 µl final volume while using the same amount of DNA and enzyme.