Labo intégré en sciences de la vie I

BIO-203

WATCH Standard Protocol Restriction Enzyme Digest

This page is part of the content downloaded from WATCH Standard Protocol Restriction Enzyme Digest on Wednesday, 25 December 2024, 22:15. Note that some content and any files larger than 50 MB are not downloaded.

Description

For optimal digestion it important to use the correct type of mixing.

Questions:

  • What are the two types of mixing recommended for the reaction?
  • What type of mixing is NOT recommended?
To save material, we scaled the reaction volume down to 20 µl final volume while using the same amount of DNA and enzyme.