Labo intégré en sciences de la vie I

BIO-203

WATCH Plasmid DNA Purification using QIAprep Spin Miniprep Kit and Vacuum Manifold

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Description

For optimal quality and yield it important to use the correct type of mixing (vortex or invert tube 4-6x) during the different steps.

Questions:

  • How do you resuspend the bacterial pellet in buffer P1?
  • How do you mix after addition of alkaline lysis buffer P2? 
  • How do you mix after addition of neutralization buffer N3?
We will use a microcentrifuge instead of vacuum, the principle is the same. No lyse blue dye was added and thus the lysis will not turn blue.