Labo intégré en sciences de la vie I

BIO-203

COMPLETE Benchling Exercises 1+2 of Lab 3

This page is part of the content downloaded from COMPLETE Benchling Exercises 1+2 of Lab 3 on Wednesday, 25 December 2024, 22:15. Note that some content and any files larger than 50 MB are not downloaded.

Description

Walk-in support sessions for Benchling Exercises

Tuesday 15.10 14:15-15:00 CO 121
Friday 18.10 15:15-16:00 CO 121
Tuesday 29.10 14:15-15:00 CO 121
Friday 01.11 15:15-16:00 CO 121


To learn the basics of virtual cloning, complete Benchling exercise 1 and 2 using this step-by-step manual and sequences provided below.Exercise 1 primers

  • Mm-Amy2_for: CAGGATCCACCATGAAGTTCGTTCTGCTGCTTTC
  • Mm-Amy2_rev: GGCTCGAGTTCATACAATTTTGAGTCAGCATGGATTG

Exercise 1 vector sequence pcDNA6/myc-His A


Exercise 2 primers

  • Mm_Amy2_HindIII_F: CGAAGCTTACCATGAAGTTCGTTCTGCTGCTTTC
  • Mm_Amy2_NotI_R: ATGCGGCCGCCCATACAATTTTGAGTCAGCATGGATTGC

Exercise 2 vector sequence pcDNA6/myc-His B


Files and subfolders